Physical exercise prevents behavioral alterations in a reserpine-treated zebrafish: A putative depression model.

Major depressive disorder (MDD) has increasingly reached the world population with an expressive increase in recent years due to the COVID-19 pandemic. Here we used adult zebrafish (Danio rerio) as a model to verify the effects of reserpine on behavior and neurotransmitter levels. We observed an increase in the immobile time and time spent in the bottom zone of the tank in reserpine-exposed animals. The results demonstrated a decrease in distance traveled and velocity. Reserpine exposure did not induce changes in memory and social interaction compared to the control group. We also evaluated the influence of exposure to fluoxetine, a well-known antidepressant, on the behavior of reserpine-exposed animals. We observed a reversal of behavioral alterations caused by reserpine. To verify whether behavioral alterations in the putative depression model induced by reserpine could be prevented, the animals were subjected to physical exercise for 6 weeks. The results showed a protective effect of the physical exercise against the behavioral changes caused by reserpine in zebrafish. In addition, we observed a reduction in dopamine and serotonin levels and an increase in the 3,4-dihydroxyphenylacetic acid (DOPAC) levels in the brain. Physical exercise was able to prevent the changes in dopamine and serotonin levels, reinforcing that the preventive effect promoted by physical exercise is related to the modulation of neurotransmitter levels. Our findings showed that reserpine was effective in the induction of a putative depression model in zebrafish and that physical exercise may be an alternative to prevent the effects induced by reserpine.

Two Years of COVID-19 Pandemic: How the Brazilian Serie A Championship Was Affected by Home Advantage, Performance and Disciplinary Aspects.

As a result of the COVID-19 pandemic, the Brazilian Serie A championship was played without crowds in 2020 and partially in the 2021 season. We verified if the home advantage (HA) was different between the 2018, 2019, 2020, and 2021 seasons. We also compared the HA, performance, and disciplinary aspects between the rounds with or without crowds and verified the association between the number of absent athletes because of health protocols and the HA in the 2020 and 2021 seasons. We calculated the HA using the Pollard method. The performance aspects were goals, corners, shots, and ball possession, and the disciplinary aspects were fouls, yellow cards, and red cards. The HA was higher in the 2018 season compared with the other seasons. The rounds with crowds showed higher HAs than the two previous seasons and the teams had more shots and scored more goals than in the rounds without crowds. There were 457 athletes in the 2020 season and 123 athletes in the 2021 season who were absent because of health protocols, and there was no association between absence and HA. The COVID-19 pandemic affected soccer in the two last seasons in different ways in the Brazilian Serie A championship.